Immuno Services


NEMOD offers a broad range of services in state-of-the-art techniques to test and optimize cellular and humoral immune responses in vitro and in vivo. If you are looking for outsourcing opportunities to make your research efforts more effective, make use of our know-how and experience.

We offer customized solutions for the individual needs of our customers and our services are performed to the highest quality and documentation standards.

Technologies:
  Phenotypic Analysis of Blood & Tumor cells more
  In Vitro and In Vivo Functional Analysis:
  T-Cells & Dendritic Cells		 more
  Antigen Loading, Processing and Presentation more
  In Vitro and In Vivo Functional Analysis:
  Macrophages and NK cells		 more
  Phenotypic Analysis: Antibody Responses more
  In Vitro and In Vivo Functional Analysis: Antibodies more

Applications:
  T-Cell Epitope Identification, Validation and Optimization more
  Analysis/ Screening for influence of drug candidates
  on the cellular and humoral immune system		 more
  Analysis/ Screening for allergic potential of substances more
  Quality Control more
  Immunomonitoring more



More information:
If you are interested in more information or would like to get a customized Immuno-Service offer, please contact Hans Baumeister or our Immuno-Service hotline at:
E-mail:
Telephone:  
Fax:
 immuno.service
+49-30-941084-207
+49-30-941084-188

You may download a file with all the information here



Immuno-Service in detail:
In more detail, our Immuno-Service platform encompasses the following technologies and may be used for the following applications:

Technologies:
Phenotypic Analysis of blood & tumor cells
  Flow cytometry
  Tetramer staining of T-cells
  Immuncytochemistry
  RT-PCR
  Array hybridization (Affymetrix chip-based)
Functional Analysis: T-Cells & Dendritic Cells
  ELISPOT analyses
  Cytotoxicity experiments
  Tetramer staining of T-cells
  Generation of T-cell clones
  Cytokine profiling
  Mouse model: vaccination of NOD-SCID mice with human dendritic cells
Antigen Loading, Processing and Presentation
  Peptides, proteins and cell lysates
  Transfected dendritic cells with DNA or RNA
  Dendritic cells fused with tumor cells
Functional Analysis: Macrophages and NK cells
  Binding assays
  Phagocytosis assays (microscopy- and flow cytometry-based)
  Chromium and Europium release assays
  Apoptosis induction
  Cytokine profiling
  Mouse model: tumour graft models for macrophage or NK mediated killing
Phenotypic Analysis: Antibody Responses
  Immunization of mice (immuncompetent, nude, NOD-SCID recomplemented
     with human PBMC) with antigens
  Determination of antigen specific antibody responses
  Isotype determination
  Determination of antibody levels in human sera
  Determination of antigen and antigen-antibody complexes in sera
Functional Analysis: Antibodies
  Epitope mapping (e.g. peptides, carbohydrates, glycoproteins,
     phosphorylated epitopes)
  Affinity determination
  Test for internalization of antibodies (toxin- and microscopy-based)
  Antibody mediated target cell lysis by NK cells or macrophages (ADCC)
  Antibody mediated phagocytosis by macrophages
  Determination of ADCC mediated cytokine profile
  Determination of induced apoptosis
  CDC tests (complement mediated cytotoxicity)
  Mouse models: biodistribution, MTD, tumour challenge and therapy models


Applications:
T-Cell Epitope Identification, Validation and Optimization
  Applicable in various areas, e.g. oncology, allergy, autoimmune diseases
  Techniques available:
      Loading of dendritic cells with antigen of interest, e.g. proteins or peptides
      from libraries, from transfected DC (DNA, RNA), from cell lysates or cell
      fusions
      Sceening by T- and dendritic cell cytokine profiling
      Sceening by ELISPOT analysis with defined peptides
      Screening of mutagenized epitopes for optimization
Analysis/ Screening for influence of drug candidates on the cellular
and humoral immune system
  Measurement of influence of substances on:
      DC differenciation and maturation
      DC processing and presentation of antigens
      DC activation of T-cells
      DC activation of NKT cells
          Skewing into DC type 2 vs DC type 1
      Induction of TH1 vs Th2 responses
      Phenotype and function of macrophages and NK cells
      Antibody production (titres, isotypes, specificities)
  In vitro analysis in various assys
  In vivo analysis using NOD-SCID mice reconstituted with human PBLs
Analysis/ Screening for allergic potential of substances
  Measurement of influence of substances on DC development and T-cell activation:
      Skewing into DC type 2 vs DC type 1 in combination with cytokine profile
      Induction of Th2 responses by cytokine release
  Screnning of:
      Biologicals and pharmaceuticals
      Food ingredients
      Cosmetics
Quality Control
  Standardized immature and mature DC for flow cytometry control
  Standardized control for differentiation and maturation of dendritic cells
  Standardized control for T-cell activation
  Standardized control for antigen processing and presentation
Immunomonitoring
  Characterization of specific T-cell activation:
      ELISPOT analysis
      T- and DC cytokine profiling
      Tetramer staining
      Cytotoxicity experiments
      T-cell cloning
  Characterization of humoral responses
      Determination of antigen specific antibody responses
      Isotype determination
      Determination of antibody levels in human sera
      Determination of antigen and antigen-antibody complexes in sera